纤维粘连蛋白 meaning in English
fn:fibronectin
Examples
- But in serum cell culture system , azphpia - pst surface could not detach cells completely . two typical serum proteins were used to find the reason
用两种典型的血清蛋白(牛血清白蛋白bsa和纤维粘连蛋白fibronectin )模拟复杂的血清系统，研究血清蛋白对细胞脱附的影响。 - Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells . only after adhering to surface followed by spreading can cells develop and proliferate . most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity , which may correlates to the low degree of cell attachment . modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation . cell attachment to substrate is primarily mediated by integrins , a widely expressed family of heterodimeric surface receptors . most extrcellular matrix proteins such as fibronectin , osteopontin , collagen type i , bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin . the main aim of this research is to measure , assess adhesion , proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin , collagen type i or biotie gen , which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture . ( 2 ) adhesion , proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed . ( 3 ) also , adhesion , proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin , or collagen type i respectively . ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo . this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities
因此将这些蛋白包被、固定到材料表面，观察骨组织工程种子细胞mscs细胞的粘附、生长特性是本研究的中心环节，并从以下方面进行探讨： ( 1 )采用不同原代细胞分离方法，研究其对mscs细胞的生物学特性影响。 ( 2 )检测基因胜肽胶对mscs细胞粘附、增殖及分化的影响。 ( 3 )分别采用型胶原及纤维粘连蛋白( fibronectin ， fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycolide ) ， plga )膜及多孔块型plga材料，观察细胞在单层或三维培养状态下，型胶原及fn对mscs细胞粘附、增殖及向成骨细胞分化效应及能力。 - There was no difference in other biologic characteristic of mscs between the two separation method , such as cell anchorage ratio and clone formation ratio . ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ) . big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate , around the big aperture there were many round micropores about 5 m size . all of the structure were equal and uniform , which satisfied the further research work . ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h , cell number was ( 1 . 5 0 . 18 ) 105 in the plga film coated with biotiegen group , which was significantly higher than that in plga film group ( p < 0 . 01 ) and higher than that in coverslip group ( p < 0 . 05 ) , which cell number was ( 1 . 04 0 . 21 ) 105 . after 6h and 12h biotiegen could not promote cell adhesion , and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days . ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型胶原、纤维粘连蛋白促进细胞增殖，细胞接种后3 、 6 、 gd三个检测时间点，实验组细胞均明显高于对照组。与1型胶原相比，纤维粘连蛋白刺激作用更强。 ） i型胶原、纤维粘连蛋白尚能诱导mscs细胞向成骨细胞分化，不仅表达成骨细胞标志物ocn 、 alp 、 opnmrna ，而且碱性磷酸酶活性明显增高，碱性磷酸酶及钙结节7第四军医大学博士学位论文一染色均强阳性， i型胶原组mscs细胞碱性磷酸酶活性较fn组更高，有显著性差异；同时，兔疫组化染色表明，经纤维粘连蛋白作用的mscs1型胶原表达阳性。