粗分级 meaning in English
rough classification
Examples
- After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography , the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants
表达菌甲醇诱导发酵；发酵上清液经超滤、硫酸胺粗分级分离以及阴离于交换层析，纯化出ctla4胞外区蛋白。 - Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae , extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates . the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents . a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用线虫诱导下owvt - 1菌株液体发酵，通过粗分级分离、离子交换层析和凝胶过滤层析分离提纯了一个分子量为31kda的丝氨酸蛋白酶，生物学测定表明其对大豆胞囊线虫二龄幼虫具有致死作用，同时测定了该酶理化特性，酶活力在75附近酶活力最高，随着ph的增加酶的稳定性升高，与胆碱酯酶具有相似的ph曲线，对特异性底物aape ( suc - ala - ala - pro - glu - pna )具有作用， ssi和ci - 2抑制该酶的活性。