简并引物 meaning in English
degenerate primer
degenerate sequence
Examples
- Detection of lily mottle virus by rt - pcr using special primers and degenerate primers
应用特异引物和简并引物检测百合斑驳病毒 - Automated programming of degenerate primers and the cloning of the diamondback esterase gene
程序化设计简并引物与克隆小菜蛾酯酶基因 - Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae . six novel rip gene fragments ( 408 ' bp ) , 1 from benincasa hispida and 5 from cucurbit a moschaia
根据葫芦科rip上、下游两段高度保守的氨基酸序列设计简并引物对ly1 ly2 ，对基因组dna进行pcr扩增，首次建立了rip新基因快速筛选体系。 - Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
利用genbank中已经登录的其它植物中该酶基因的保守序列，设计一对简并引物，采用rt - pcr技术，从茶树扩增出208bp的cdna片段。 - These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells . two degenerate oligoxynucleotide primers ( c8 - 2 , c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8
根据嗜盐菌素cs的n端氨基酸序列设计了一对简并引物( cs一2 ， cs礴) ，利用这对引物从as7002的总dna中扩增出halcs的部分序列。