简并寡核苷酸 meaning in English
degenerate oligonucleotide
Examples
- All kind of oligonucleotides ( 16 sense primers , 8 antisenset primers , 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna . no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations
依据nbs - lrr类r基因特有的两个保守序列glpl和cfly (两者相距约70个氨基酸)合成所有可能的简并寡核苷酸引物(左引物16个,右引物8个,共组成128个引物组合)对玉米基因组dna进行扩增的结果表明:在6个引物组合中得到了扩增产物,产物长度在500 1000bp之间;没有获得特异性扩增产物(指仅从含ht基因材料中得到,长度约210bp的dna片断) 。