穿梭载体 meaning in English
shuttle vectors
Examples
- The expression plasmid called psugv - badfe was constructed by inserting ba - dfe gene into e . coli - b . subtilis shuttle vector psugv4 and the
将ba0fe基因克隆到大肠杆菌一枯草杆菌穿梭载体psugv4中，得到重组质粒psugv badfe ，然后转化到枯草杆菌wb600中进行了分泌表达。 - With the aim of increasing the expression and stability of mtxl , the mtxl gene originated from b . sphaericus ssii1 was cloned to a shuttle vector pbu4 . two recombinant plasmids pmt9 and pmt4 were obtained , with the inserted fragments in the opposite orientations
为了提高mtx1杀蚊毒素蛋白的表达量和稳定性，本文将来源于球形芽孢杆菌b . sss - 1的mtx1毒素基因克隆至穿梭载体pbu4上，得到mtx1插入方向相反的重组质粒pmt9和pmt4 。 - The sacvgb gene was then cloned into e . coli - - b . sublilis shuttle vector psugv4 and a recombinant plasmid named psu - sacvgb was constructed . after transformation with b . sublilis wb600 , the transformants successfully produced vhb intracellularly . the influence of vhb on b . subtilis is under investigation
将sacvgb基因克隆到大肠杆菌-枯草杆菌穿梭载体psugv4中，获得重组表达质粒psu - sacvgb ，然后转化枯草杆菌wb600 ，转化子经2蔗糖诱导，该基因在枯草杆菌中表达。 - In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ) , and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee . the plasmid pfb 1 - cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies , which was recombinant baculovirus shuttle vector containing hcv structural gene , named bac - cee
本实验用pcr扩增hcv结构区基因，克隆到杆状病毒表达载体pfastbacl中，构建成重组转座载体pfb1 - cee ，转化dh10bac大肠杆菌感受态细胞，筛选阳性菌落，抽提大分子质粒dna ，获得含hcv结构区基因的重组杆状病毒穿梭载体bac - cee ，脂质体介导转染sf9昆虫细胞，出现细胞病变后，收集含有重组杆状病毒颗粒的培养上消，重新感染sf9细胞，收集sf9细胞，进行12 . 5 sds -聚丙烯酰胺凝胶电泳，可见表达的蛋白条带。