禽痘病毒 meaning in English
avian variola virus
fowl avipoxvirus
fowlpox virus
Examples
- In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
本研究采用脂质体转染方法，将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞，经6轮蚀斑克隆、筛选、表达， pcr鉴定和dot - elisa检测，证明该重组病毒已构建成功，并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。 - Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538 , and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid . both vp3 gene and lacz gene were cloned into noti site of psy681 , recombinan fpv transfer vector containing vp3 gene of gpv was obtained . the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另从含有gpvh1分离株主要结构蛋白vp3基因的重组质粒pproexhtb - vp3中切取gpvh1株vp3基因片段，将其亚克隆于psy538的ecori位点，然后将带有痘苗病毒启动子p11的lacz报告基因平端克隆于上述重组子的smai位点，再用noti切下同时含有vp3基因和lacz报告基因的片段，再亚克隆于psy681的noti位点，构建出含有vp3基因的重组禽痘病毒转移载体，为构建表达vp3基因的重组禽痘病毒从而制备gpv基因工程疫苗奠定了基础。 - The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr , and express by protokaryotic and eukaryotic expressing system , then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine
利用pcr方法扩增和克隆gpvh1分离株主要免疫原性蛋白基因vp3 ，并对其进行原核和真核表达，是建立小鹅瘟分子诊断方法、构建vp3基因重组禽痘病毒活载体疫苗的基础，具有极为重要理论和实践意义。