磷酸胆碱 meaning in English
choline phosphate
pho horylcholine
phosphocholine
phosphorylcholine
Examples
- The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 - Methods : in cultured lung explants without serum , the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity , nitric oxide ( no ) content , superoxide dismutase ( sod ) level , malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods . results : 1 . influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine , glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry , glu induced the changes of the ultrastruture of at ii iv cells
方法：采用成年大鼠肺组织无血清培养，运用[ ~ 3h ] -胆碱掺入法测定ps主要脂质磷脂酰胆碱( pc )合成量； rt - pcr扩增检测肺组织中pc合成限速酶磷酸胆碱二胞苷酰基转移酶( cct ) mrna含量；透射电子显微镜法观察肺泡型上皮细胞和ps系统超微结构的变化；免疫组织化学染色检测glu的受体nmdar1亚单位的表达；生化测定肺组织乳酸脱氢酶( ldh )释放量和肺组织匀浆中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。