硫酸铵沉淀 meaning in English
ammonium sulfate precipitation
Examples
- An antifungal protein , named as b16 , was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate , desalt and gel filtration on sephadex g - 75 , which with molecular weight at about 30 - 40 kd on the basis of sds - page
菌株041381发酵上清液经70饱和度硫酸铵沉淀，透析脱盐， sephadexg - 75凝胶过滤层柱，得到活性物质b16 。以sds - page胶为基础进行电泳分析， b16的分子量为30 - 40kd 。 - Without ammonium sulphate fractionation and dialysis , the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l , ph7 . 8 ) , and the enzyme fraction was not absorbed on the column but impurities were absorbed
粗酶液无需硫酸铵沉淀及透析，即可引入磷酸缓冲液( 50mmol l ， ph7 . 8 )预平衡的deae - sepharosecl6b柱，上柱后用平衡缓冲液洗至基线稳定。 afpga不被吸附而直接流出。 - We have sifted 103 medicinal plants , roughly identified 17 plants might contain antifungal proteins . antifungal protein was purified from cassia sophera linn , by extraction , fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ) , cation - exchange chromatography of cm - sepharose ff xk 26 , the first cation - exchange chromatography of mono s and the second one , followed by gel filtration of superose 12hr
对茳芒决明进行了抗菌蛋白的分离纯化：经粉碎、磷酸缓冲液浸提、硫酸铵沉淀、 cm - sepharoseffxk26阳离子交换层析、两次monos阳离子交换层析、 superose12hr分子筛层析可得到具抗真菌活性的蛋白。 - The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation , deae - sepharose fast flow ion - exchange chromatography , and sephacryl column gel - filtration , and its biochemical and enzymological properties were identified in this study . it was found that the deduced molecular weight of he in sds - page is about 98 . 5 kda , and its proteolytic activity was optimized at ph of 7 . 5 - 8 . 5 and at temperature of 40 , respectively
我们利用67硫酸铵沉淀、 deae - sepharosefastflow阴离子交换柱层析和sephacryl凝胶过滤柱层析，并以酪蛋白为其蛋白酶水解活性的检测底物、以卵膜为其卵壳裂解活性的特异性底物，从卤虫胚胎孵化液中分离纯化出了卤虫的孵化酶分子，其在sds - page电泳中的分子量约为98 . 5kda 。