硫氰酸胍 meaning in English
guanidine isothiocyanate
guanidine thiocyanate
guanidinium thiocyanate
Examples
- The main results are as below . 1 . we extracted rna from narcissus , cloned the cdna sequence of chs gene by rt - pcr and analyzed the coding sequence of gene
主要结果如下： ( 1 )以即将开放的中国水仙的花蕾为材料，采用异硫氰酸胍-苯酚-氯仿法提取rna ，反转录成cdna后用特异引物pcr扩增出1 - In the experiment , the first instar housefly larvae " metallothionein mrna expressing reach maximur . after induced by cd ( 2mm ) for 48 hours , then total rna was extracted with guanidinium isothiocyanate , and mrna was isolated by the polyattract mrna isolation systems surplied by promega company
试验中采用2mmcd ~ 2对家蝇初孵幼虫诱导48小时，使其体内金属硫蛋白mrna表达达到高峰，在液氮中将其研磨成粉末后采用异硫氰酸胍法提取总rna ，再用promega公司提供的小量分离mrna试剂盒纯化mrna 。 - In this research , total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ) . based on reported sequence of hsa cdna , specific primers were designed to amplify its encoding region by rt - pcr . at the same time , kozak sequence was added to the translation site , to strengthen initiation of translation
本研究通过改进的异硫氰酸胍-酸性酚-氯仿一步法从人工流产胎儿肝脏中提取总rna ，然后根据报道的序列设计特异性引物，同时在上游引物中引入了kozak序列，经rt - pcr克隆到hsacdna编码区序列，长度为1758bp 。 - The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method , then the total rna digested by dnase that had not rnase was used for rt - pcr . i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition . at the end i selected the magnesium ion density as 1 . 25 mm . the production of rt - pcr was inserted directionally into pgem ? z ( ampr ) . the pgem ? z ( ampr ) was used to transform e coli jm109 . i got a positive clone through culling and identificatin . the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取诱导培养液中的菌体，用异硫氰酸胍法提取总rna ，总rna再经无rna酶的dna酶处理后用于rt ? pcr 。在pcr扩增目的基因时，通过优选扩增体系，使镁离子浓度为1 . 25mm时rt ? pcr可顺利地获得目的基因，并能定向克隆到载体pgem ? 3z ( amp ~ r )中。用克隆载体转化宿主大肠杆菌jm109 ，通过筛选获取阳性克隆子，对阳性克隆子进行酶切与pcr鉴定，并对载体中插入的目的基因进行测序。 - Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish . the construction and evalution of the a , gtlo cdna library of grass carp leukocytes are described . total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction
本文第一部分取病毒感染36小时后的草鱼头肾并分离白细胞，用改进的异硫氰酸胍一步法从其中提取总rna ，磁珠法分离纯化mrna ，电泳检测其质量。