短小芽孢杆菌 meaning in English
bacillus pumilis
Examples
- Bacillus pumilus un - 31 - c - 42 was obtained by mutations of strain ba ( 06 ) , which is an alkaline protease producer and separated from life waste in chengdu
短小芽孢杆菌( bacilluspumilus ) un - 42 - c - 31是一株从成都市生活垃圾中分离，经过多次反复诱变后得到的碱性蛋白酶产生菌。 - The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues , which is part of a alkaline serine protease , with 98 . 3 % homogeneity to a reported gene from bacillus pumilus
序列分析显示，该片段是一个全长1106bp的orf ，可编码369个氨基酸残基的多肽，与短小芽孢杆菌碱性蛋白酶基因编码区高度同源。 - A pair of primers , based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus , were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf对酶活力有不同程度的抑制作用。以4株芽孢杆菌的总dna为模版，利用扩增短小芽孢杆菌碱性蛋白酶基因的引物对它们进行pcr扩增。 - The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp . the fermentation liquor also showed good dehairing activity . the alkaline protease ( named dhap , dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography , ion exchange and gel filtration
通过cm - sepharosefastflow离子交换层析， deae - sepharosefastflow离子交换层析， sephacryls - 100 ， sephacryls - 200凝胶过滤层析，疏水层析等纯化步骤对短小芽孢杆菌发酵液中的碱性蛋白酶进行了纯化。 - High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria , which was shown as follows : firstly , physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography . for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography . chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied
本文利用高效液相离子交换色谱系统研究细菌学，探讨了该方法在细菌定性、定量方面的应用，主要包括三个方面：首先，利用离子交换色谱系统表征细菌生理、生态方面的变化，首次成功地在色谱上区分了短小芽孢杆菌的芽孢及营养体；考察了不同的培养环境对细菌色谱行为的影响及不同生长阶段的细菌的色谱行为。