盲传 meaning in English
blind passage
Examples
- Ma 104 cells were used for the replication and isolation of the virus . after four passages , the isolate could produce obvious cytopahic effects ( cpe ) . typical rotavirus particles were seen with direct electron microscope
本实验从吉林省某猪场发生腹泻的猪群粪样中，用ma104细胞培养，分离到一株prv ，盲传至4代出现明显病变，经电镜观察见有典型的车轮状病毒颗粒。 - In this study , one strain of avian rotavirus was isolated from a chicken with natural diarrthoea . isolation and cultivation of the rotavirus was attemped by the inoculating on monolayers of marc 145 cells with a suspension of faecal material containing the rotavirus
本文对发生在山东某地鸡流行性腹泻病例的病原进行了研究。用胰蛋白酶处理病鸡的粪便和肠内容物，接种marc145细胞，盲传数代后分离到一株病毒。 - Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area , in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china , thus providing theoretic basis for the study of vaccine against it . the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages . when the cpe was obvious , the virus was harvested and purified
为了弄清我国不同地区prrsv的来源以及其遗传学背景，为疫苗学研究提供理论根据，本研究在ch - 1a株完整的基因组获得以后，从流行于我国山东( sd )和黑龙江大庆( dq )地区疑似prrs的猪体内分离到prrsv ，在mark - 145细胞上盲传5 6代，细胞出现明显病变以后，收获病毒液，然后提纯，提取全病毒rna ，经过反转录、 pcr扩增获得结构基因orf2 7的目的基因片断，然后与pmd - t载体连接，转化，得到阳性质粒后进行测序，并将其与ch - 1a株进行了比较分析，同时对这两个毒株的结构基因组的理化性质进行分析。