疏水氨基酸 meaning in English
hydrophobic amino acid
Examples
- Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e . coli bl21 ( de3 ) . no squalene synthase polypeptide of expected molecular mass was observed in e . coli containing the putative full - length squalene synthase cdna , however , overexpression in e . coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全长的鲨烯合酶cdna的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而c末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。 - Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ) , which encoded a protein consisting of 524 aa with molecular weight of 62 . 2 kda and pi of 8 . 96 . strongly basic ( + ) amino acids , strongly acidic ( - ) amino acids , hydrophobic amino acids and polar amino acids of the protein were 13 . 74 % , 11 . 64 % , 36 . 45 % and 22 . 70 % respectively , and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site , protein kinase c phosphorylation site , casein kinase ii phosphorylation site , n - myristoylation site , camp - and cgmp - dependent protein kinase phosphorylation site , tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450 . a - helix and b - sheet of the protein is 47 . 7 % , 45 . 0 % respectively
Huangya14 )为材料分离克隆到一个细胞色素p450基因，命名为bccyp86mf5 ， cdna全长1854bp ，含1575bp的完整开放阅读框，编码524个氨基酸，其编码蛋白质的分子量为61 . 2kda 、等电点为8 . 96 ；碱性氨基酸、酸性氨基酸、疏水氨基酸和极性氨基酸分别占总氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二级结构预测包括n -糖基化位点、依赖于camp和cgmp的蛋白激酶磷酸化位点、蛋白激酶c磷酸化位点、酪蛋白激酶磷酸化位点、酪氨基酸激酶磷酸化位点、 n -豆蔻酰化位点和细胞色素p450的典型区域，半胱氨酸亚铁血红素配体信号区等， -螺旋和-折叠分别占47 . 7 、 45 . 0 ；与bccyp86mf1基因的氨基酸序列同源性达到95 . 2 ，与拟南芥cyp86c4的达到85 . 9 。