电泳迁移率改变 meaning in English
emsa
Examples
- 6 . emsa with coincubation of specific antibodies to the p50 or p65 subunits of nf - b showed a distinct retardation in the mobility with the p65 antibody and a reduction in the intensity of the shifted band with the p50 antibody
6 ，加入抗nf一kbp65和p50的抗体进行电泳迁移率改变实验，证实nf一kb形成p50 / 65异二聚体参与mbd3基因的转录调节。 - In order to study the expression of 3 - defensins in liver as acute phase response proteins , a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study . the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps . the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探针，经[ - ~ ( 32 ) p ] atp标记后通过northernblot方法检测mbd3在肝脏中的表达，同时分析了mbd3基因诱导表达的组织特异性，剂效和时效关系；结合mbd3基因启动子区序列分析，以- 164 ? - 179bp双链dna序列合成探针，经[ - ~ ( 32 ) p ] dctp标记后通过电泳迁移率改变实验( emsa )和south ? westernblot方法对参与mbd3在肝脏中诱导表达调节的转录因子进行分析。 - The results of emsa showed the obvious retardant bands , which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment . 5 . the molecular mass of this dna binding protein was assessed between 43 . 0 - 66 . 2 kd by south - western blot
4 .电泳迁移率改变实验有明显的滞后带形成，表明lps刺激后，肝脏组织的细胞核内有转录因子与mbd3基因特异的dna序列结合，参与mbd3基因的诱导表达5 . south一westemblot进一步确定此转录因子分子量在43 . 0一“ . 2kd之间。