电压钳 meaning in English
voltage clamp
Examples
- Under the whole - cell patch - clamp mode , we further observed the modulation of sodium channels on adult rat dorsal root ganglion ( drg ) neurons by hntx - iv and hntx - v
在全细胞电压钳记录模式下,我们进一步观察了两毒素对sd大鼠背根神经节( drg )细胞电压门控激活钠电流( i _ ( na ) )的影响。 - The pipette was positioned on a neuron using a hydraulic micromanipulator by blind prick technique . after a giga fi seal formation , the whole - cell recording was performed by an axopatch 200b amplifier
以水压式微操纵器采用盲扎法将电极置于待测神经元上,形成巨阻封接后,用axopatch200b放大器做全细胞电压钳记录。 - At first , the vrl - cdna plasmids were transformed into tg1 r . coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently , vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro , following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp
这项实验中,我们首先将大白鼠vr1 - cdna质粒转入大肠杆菌中进行扩增,然后提取质粒并酶切,然后在体外转录成vr1 - mrna ,接着将vr1 - mrna注射到非洲爪蟾卵母细胞中表达为蛋白质受体,建立了实验模型细胞,最后用双微电极电压钳检测此模型细胞。