特异性抗血清 meaning in English
specific antisera
Examples
- Synthesized the peptide according to the sequence of ast7 . the peptide was linked with carried protein bsa and then was used as fusion antigens for immunization
并根据它的氨基酸序列合成小肽，与载体偶联后免疫家兔诱导产生特异性抗血清。 - This modification includes : ( 1 ) selecting two important molecules as candidates , ( 2 ) choosing a promiscuous t - cell epitope , and two b - cell epitopes or conserved amino acid sequences from the two important molecules , ( 3 ) connecting them adequately through analysis by the molecule designing software . therefore , the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing . based on the molecule designing methods , the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse , as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白质分子设计的理论和方法研究避孕疫苗，将sp17和cyritestin关键表位和牛核糖核酸酶非选择性th细胞表位合理组合，获得新抗原- 35肽序列；并在合成、纯化后分别与弗氏佐剂、免疫刺激复合物( iscoms )混合后免疫不同遗传背景的雌性小鼠，观察血清和生殖道内的特异性抗体滴度的动态变化、生育力的改变以及免疫后小鼠重要脏器的组织病理学改变：以及在ivf下，新抗原的特异性抗血清对精卵相互作用的影响及抗原在精子表面的特异性定位。 - Northern blot analysis indicated that foreign ibdv vp2 gene was correctly transcripted into mrna . the recombinant protein from tobacco leaves was detected by dot - elisa and western blot using antisera specific for ibdv . the protein ibdv vp2 expressed in tobacco appeared with apparent molecular mass of 52kd
取生长期烟草植株，经pcr检测和southern杂交检测，证明外源基因ibdvvp2成功整合到烟草染色体上； northern杂交分析表明转基因烟草中存在正确转录的ibdvvp2基因的mrna ； dot - elisa和westernblot分析证实转基因烟草中产生了能与ibdv特异性抗血清反应的ibdv抗原蛋白，其大小约为50kd 。 - The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene , and the alignment of the amino acid sequence was 98 % . the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e . coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition , and exhibited about 62kda in size , very close to the predicted molecular weight of gst - momp . furthermore , the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page电泳分析显示诱导表达的基因产物分子量约为62kda ，与预测的gst -外膜蛋白重组融合蛋白的分子量极为相似， western - blot进一步证实，表达产物能被嗜水气单胞菌l316主要外膜蛋白特异性抗血清所识别，产生明显的染色条带，说明所表达的基因产物与天然的外膜蛋白抗原性一致。 - From dead chickens , one virrus was isolated by using eggs and chicken embryo fibroblast . lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum . this strain ' s biological property was tested by barren spot , cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本实验采用spf鸡胚及鸡胚原代成纤维细胞，从河北省某鸡场新城疫免疫抗体很高的病死鸡的脑组织中分离得到一株病毒。此株病毒能凝集鸡的红细胞，并且这种凝集可以被特异性抗血清所抑制。