淋巴毒素 meaning in English
lymphotoxin (lt)
Examples
- Promote sweating and reducing subcutaneous fat , reduce lymphotoxin and deposit , and gift you a slim figure
增加排汗及促进皮下脂肪的排除；减少淋巴毒素和沉积物，窈窕瘦身。 - Researches showed that purified lta27 had the same antitumor activity with lt but far more low toxicity in vivo
初步动物实验结果表明，纯化的lt 27在体内的抗肿瘤作用与淋巴毒素完整蛋白相当，但毒副作用却小得多。 - With ni2 + - lda - sepharose , the cbd tag was removed . according to the results of sds - page and western blot analysis , the product of outflow was the target protein lt a 27 . the purity of lt a 27 was about 95 %
运用niz +金属鳌合亲和层析柱进一步快速纯化去除融合头，上样流出经sds一队ge分析和western印迹分析ii1 :明得到的纯化产物为淋巴毒素缺失体蛋白lt凸27 ， lt 2 :产物纯度可达95 %以上。 - The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target , lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt . the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1 . 6 0 . 1 104 the dosage of amd was lug / ml , and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution . the credibility of the established system was detected with rhtnfp developed by r & d
为确定经上述步骤纯化后得到的目的蛋自lt 27的生物活性，本研究以l929细胞为靶细胞、淋巴毒素国际标准品为参照，采用结晶紫染色法检测经淋巴毒素处理后存活的细胞，对淋巴毒素生物活性测定的细胞接种浓度、淋巴毒素标准品板上稀释的起始浓度和梯度稀释的倍数、放线菌素d的使用剂量等进行条件实验后，建立了人淋巴毒素生物活性测定方法。 - Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory , immunostimulatory , and antiviral responses . in order to increase the antitumor activity of lymphotoxin and reduce its side effects , the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27 . the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通过构建表达n端缺失27个氨基酸的淋巴毒素融合蛋白的重组质粒，在大肠杆菌中实现融合蛋白的可溶及分泌表达，同时利用表达载体上的几种特殊序列经简单的分离纯化步骤直接获得大量的有生物活性的淋巴毒素缺失体lt 27 ，为寻找一种高抗肿瘤活性、低临床毒副作用的生物抗癌药物进行了有效的探索。