消减杂交 meaning in English
suppression subtractive hybridization ssh
Examples
- In previous study , janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization , and registered in genbank
在前期的研究工作中，本室姜宏等通过建立小鼠隐睾模型，运用抑制消减杂交法( suppressionsubtractivehybridization ， ssh )克隆出24个小鼠睾丸生精细胞凋亡相关基因的est ，并在genbank中登录。 - The results from rt - pcr of the three differentially expressed genes were significantly higher than those of control group ( p < 0 . 01 ) in summary , with rapidness and effectiveness of ssh technique , the screened differentially expressed genes including the known and three novel ones in this study might play an important role in the pathogenesis of rat brain injury induced by repeated + gz exposures
选取的3个差异表达基因用半定量rl 、 pcr检定结果表明，各基因在对照组和暴露组中的表达水平有明显差异( p < 0 . 01 ) 。上述研究结果提示，应用抑制性消减杂交这一筛选差异表达基因的快速有效工具所发现的3个新的cdna ，可能在+ gz脑损伤的病理过程中起重要作用。 - In this study , sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group . the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury
本研究利用大鼠重复+ 10gz暴露引发脑损伤的动物模型，观察脑的病理学改变；应用抑制性消减杂交技术筛选+ gz重复暴露大鼠脑的差异表达基因，旨在初步探讨+ gz重复暴露致脑损伤的分子机制。