毒性基因 meaning in English

toxophore
virulent gene

In this study , the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi , xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing , the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli , the transformed hosts were induced by iptg , bysds - page and elisa analysis of host protein . the expression of the objective gene was detected , and it could account for 16 . 28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg
同时将原核表达载体pet - 28c用nco ， xho双酶切，回收酶切产物，将回收的酶切产物pea ， h3 ，载体进行连接，并转入dh5感受态细胞内，培养12 - 18小时后，挑取阳性菌落，经nco ， xho双酶切分析及pcr检测，筛选到阳性克隆，其质粒测序结果表明成功地构建了毒性基因缺失的pea与人组蛋白h3融合基因的原核表达载体。