柱床体积 meaning in English
bed volume
column volume
Examples
- Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation . 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies . 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs , prepare a 50 % slurry for batch purification method , pack column with matrix slurry
三、 gst一hbrp重组蛋白的纯化1 .超声破碎细胞，离心，上清和沉淀进行sds一page电泳分析2 .样品处理提取包涵体，变性后，加人用pbs平衡过的以utal肠onesepb抓脱4b ，室温下孵育3 .以utadtionesepharose4b柱纯化以utad云onesepharose4b柱的准备根据毛山lel ，决定纯化所需的gll卫ta1如oneseph娜se4b的柱床体积，用预冷的1xpbs清洗cldtathionese户， se4b ，得到50 %的基质