染色条 meaning in English

dyeing strip

The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene , and the alignment of the amino acid sequence was 98 % . the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e . coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition , and exhibited about 62kda in size , very close to the predicted molecular weight of gst - momp . furthermore , the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page电泳分析显示诱导表达的基因产物分子量约为62kda ，与预测的gst -外膜蛋白重组融合蛋白的分子量极为相似， western - blot进一步证实，表达产物能被嗜水气单胞菌l316主要外膜蛋白特异性抗血清所识别，产生明显的染色条带，说明所表达的基因产物与天然的外膜蛋白抗原性一致。