染色体步移 meaning in English
chromosal walk
chromosome walking
Examples
- The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned , sequenced and characterized by using chromoseme walking method . the sequence includes cpcb gene ( 519 bp ) , cpca gene ( 489 bp ) , cpch gene ( 357 bp ) , and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ) , 111 bp of phycocyanin intergenetic spacer ( pc - igs ) . upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e . coli , synechocystis pcc 6803 and a . platensis fachb341 by supersonic and electrophoresis methods
根据genbank中报道的节旋藻藻蓝蛋白基因序列设计引物，首先克隆了钝顶节旋藻( arthrospiraplatensisfachb341 )藻蓝蛋白操纵子中亚基基因、亚基基因部分序列及二者之间的间隔区序列( pc - igs )并进行序列测定，然后根据此测序结果设计引物，通过染色体步移法克隆得到藻蓝蛋白操纵子长度为2086bp的基因片段，其中包括藻蓝蛋白亚基基因( cpcb ， 519bp ) ，亚基基因( cpca ， 489bp ) ，连接蛋白h基因( cpch ， 357bp ) ，亚基基因上游启动子序列( 427bp )以及各基因之间的间隔区( pc - igs ， 111bp ； cpch与cpca间隔区， 184bp ) 。