杂交筛选 meaning in English
screening by hybridization
Examples
- In this paper , we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid
以dig - oligo2为探针，菌落原位杂交筛选cosmid文库，得到56个阳性重组子。 - Nor protein partner interacting with pgl29 was detected by yeast two - hybrid screening . thus further work needs to be made to understand the fu
酵母双杂交筛选结果，也未得到特异地与pgl29相互作用的直白质分于，因而现有的实验无法确定该基因的功能。 - In our study , the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones
我们将trk受体膜外域1911结构域与gal4蛋白融合构建成pgbkt7一ign ，确认无自激活作用后，将其作为诱饵蛋白，洲于酵母双杂交筛选。 - Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned
使用sau3ai对基因组dna进行不完全酶切，回收2 3kb片段，与puc18质粒连接转化大肠杆菌，利用地高辛标记探针，使用菌落原位杂交筛选转化子；筛选到包含有约1kb外源片段的转化子。 - Four c - ch3 , six aromatic carbons , six carbons of mycosamine were the characteristics of candicidin d , one ketal corresponding to mycosamine and four ketons indicated that no hemiketal formed between c - 15 and c - 19 in fr - g08b or candicidin d . however , such hemiketal was usually thought having been formed
用这个探针，对链霉菌fr - 008的基因文库进行杂交筛选，获得了3个阳性克隆，经southern杂交分析，发现它们均含有6 . 4kb共同的阳性片断。