斑点杂交 meaning in English
dot blot hybridization
dot hybridization
Examples
- Protocol of dot blot and in situ hybridization for white spot virus
对虾白斑病毒斑点杂交和原位杂交检测操作规程 - By northern dot blotting , it also showed that the foreign gene could express in the transgenic plants
Northern斑点杂交表明，外源基因在转基因植株中正常表达。 - To device a primer pairs and amplify the full length pstvd by rt - pcr , the positive rna extraction from tuber sample was used as template . the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑点杂交反应( nash )检测pstvd方法准确、灵敏度高，一次检测样品数量多，且对于异地样品检测非常方便，是以往其它检测方法的有效补充。 - Pcr , pcr - southern blot analysis , southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome . the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw , which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
( 3 )细胞核载体pb - adw 、 pbg - adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经pcr 、 pcr - southern 、 southern斑点杂交分析证实， adw基因已整合到莴苣基因组中。 - Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。