接合转移 meaning in English
conjugal transfer
conjugational transfer
conjugative mobilization
Examples
- It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s . tenebrarius
Pcr实验证明基因重组菌株中接合转移质粒同源整合到黑暗链霉菌h6的染色体上。 - The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius
通过接合转移的方法将质粒pzxb014导入黑暗链霉菌h6中,筛选基因发生重组的菌株。 - This system included method of solid medium plate enveloped with parafilm to form colony , conjugational gene transfer in a selective medium , screening the nonmagnetic mutants by magnet adsorption technique
此体系包括:以平板封膜培养技术获得单菌落,在选择性培养液中进行遗传因子接合转移,以液体培养和磁铁吸附技术筛选突变子。 - In order to facilitate the study of biological function of add gene cluster , e . coli - s . avermitilis intragenus conjugation system was established . in addition , phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed
建立了除虫链霉菌的接合转移系统,并构建了用于置换全部基因簇的基因置换质粒phz2114和adda的基因中断质粒phz2130 ,为研究除虫链霉菌add基因簇的生物学功能奠定了基础。 - Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段为探针钓出了位于该区域的文库克隆,并利用指纹印迹和杂交技术将这些文库克隆排列起来,进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒,并试图通过基因置换将该区域置换下来,但尚未得到最终结果。