持续培养 meaning in English
carrier culture
propagation
Examples
- The results show that the optimal conditions of the expression of bl21 ( de3 ) - ptxbl - hng and bl21 ( de3 ) - ptxb1 - m - insuiin are : bl medium , 37 , 200rpm for 4 . 75h , then 1ptg was added to the medium to 0 . 01mmol / l , induce for another 5h . 3 . hng fusion protein and m - insulin fusion protein are expressed as inclusion bodies in e . coli
工程菌表达条件的研究山西医科大学2003届硕士学位论文表达凿l株blzi ( de3 )一ptxbi一hng及bl21 ( de3 )一ptxbi一介insulin均在bl培养基， 37oc 、 zoorpm摇床培养4小时45分时进入对数生民中后期，此时加入终浓度0 . olmlnol / l的i尸tg开始诱导，再持续培养5小时可达到最人表达量。