抗体克隆 meaning in English
antibody cloning
Examples
- Pcr detection found there were 1 . 2x104 tfu in the library . one of colonies of scfv antibody , which binds to bull sperm , was selected and sequenced . sequence analysis indicates that the scfv colony resembles the mouse antibody
用phage - elisa从文库中筛选并鉴定出一个针对牛精子的阳性克隆，序列分析发现，该单链抗体克隆的序列符合小鼠抗体可变区的一般特征，这说明构建的针对牛精子的噬菌体抗体文库是成功的。 - The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。