密码子使用 meaning in English
codon usage
Examples
- Codon usage in genes coding for proteins with different folding types
不同折叠类型蛋白编码基因的密码子使用 - Method for the design of mrna detecting genechip based on codon usage bias
一种基于密码子使用偏性的检测型基因芯片设计方法 - Analysis of synonymous codon usage in aeropyrum pernix k1 and other crenarchaeota microorganisms
嗜热泉生古细菌及其他泉古菌同义密码子使用偏向性分析 - As a typical protein - coding gene , the second positions were least variable , first positions were more variable , and third positions were the most variable . although codon usage is effected by a / t bias , the amino acid composition is not varied with this bias
密码子以a 、 t结尾频率高，第三位是g的密码子使用较少，反映出cytb基因在密码子的使用上具有偏向性，同果蝇和蜜蜂相一致。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。