定向克隆 meaning in English
directed cloning
directional cloning
Examples
- The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
应用逆转录-聚合酶链式反应( rt - pcr )技术克隆得到编码草鱼生长激素( cgh )的基因cdna ，并定向克隆到puc18载体上。 - Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector , and e . coli bl21 was transformed by the recombinant plasmid for expression
Pcr产物经纯化、酶切后，按正确的阅读框架定向克隆到表达性载体pgex - 6p - 1中谷胱甘肽转移酶( gst )基因的下游。 - We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
利用vp7基因和质粒pbi121上相同的单克隆位点，将vp7基因定向克隆到植物表达载体pbi121上，构建了pbi121vp7表达载体。 - A plant expression vector was constructed by following method : s gene of 1bv and 35s promoter was cut from recornbinant pbi121 , and then the fragment was inserted into the multiclonal sites hind / bamh i in the plasmid pcambia1305 . 1
通过从重组质粒pbi121上切下s基因(连同35s启动子)片段，将该片段定向克隆到pcambia1305 . 1质粒的多克隆位点hind 、 bamh之间，构建了一种植物表达载体。 - For prokaryotic expression , the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ) . the gene was overexpressed in e . coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction
用pcr方法扩增番红花八氢番茄红素脱氢酶( pds )编码区序列，定向克隆到表达载体pet一艺1a ( + )上，获得重组质粒pet一21a ( + ) / cspds 。