安普霉素 meaning in English
apramycin
Examples
- To study the biosynthetics genes of apramycin in streptomyces tenebrarius , the apramycin resistant gene was isolated by gunshot cloning firstly
为了研究黑暗链霉菌安普霉素生物合成基因,首先通过鸟枪克隆的方法克隆其安普霉素抗性基因。 - The ligation mixture was used to transform s . lividans tk24 protoplasts . six colonies capable of growing in the presence of apramycin were isolated
连接液转化标准宿主菌s . lividanstk24原生质体,最终得到6个可以在安普霉素平板上生长的阳性克隆。 - Amino acid feeding experiments were also performed in this study in order to investigate the pathway , by which nitrogen incoperated into apramycin molecule , and the regulatory mechanism of nitrogen metabolism
通过氨基酸添加实验研究了安普霉素生物合成途径中的氮的来源及调节机制。 - Plasmid psyxl , containing a 12kb insert of s . tenebrarius dna , was isolated from one of six colonies . apramycin resistant gene was located in 1 . 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies
阳性克隆中重组质粒psyx1含有12kb黑暗链霉菌的dna ,对12kb片段进一步分析将安普霉素抗性基因定位在1 . 5kbsphi - kpni片段上。 - Streptomyces tenebrarius a04 , the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper . after treatment of spore suspension and mycelia shivered by supersonic with mutagen , combined with the application of screening models , some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23 , a2 - 30 , asm6 and al - 16 were obtained
本文以尼拉霉素单一组分?安普霉素产生菌s . tenebrariusa04 (发酵单位为2847u ml )为出发菌株,通过对单孢子悬液和超声波破碎菌体的诱变处理并复合筛选模型,获得了遗传特性稳定的单组分高产菌株: a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ,摇瓶发酵单位达4800 - 5200u ml 。