奎尼酸 meaning in English
acidum chinicum
quinate
quinic acid
Examples
- Metabolic engineering was introduced in the studies of recombinant e . coli constructs to produce quinic acid
我们将代谢工程技术应用于产奎尼酸基因工程菌的构建。 - Quinic acid is one of aromatic small compounds , it has widely been used as the important raw material in the fields of medicine , food and chemical industry
奎尼酸是重要的芳香族小分子化合物之一，在医药、食品、化工领域有着广泛的应用。 - Another phenolic constituent is tea , theogallin , accounts for approximately 1 percent of the dry weight of the leaf . chlorogenic acid , also a phenolic compound , is a minor constituent in tea
茶叶中另一酚类组分是3 -邻-没食子酰奎尼酸，它约占茶叶干重的1 % 。同属酚类化合物的绿原酸在茶叶中是次要成分。 - 2 . cloning and functional expression of a heterogenous gene from a . nidulans in e . coli strain a . nidulans chromosome dna was used as template to amplfy gene qutb by pcr , the dna sequencing showed that the cloned gene was in agreement with the reported sequence
异源奎尼酸脱氢酶( qdhase )在大肠杆菌中功能性表达以构巢曲霉菌( a . nidulans )基因组dna为模板，用pcr方法扩增得到了qutb基因，序列测定与文献报道一致。 - 4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction
构建宿主菌基因精确定位突变株31bk ( arod : : arobkan ~ r )为了改变代谢途径脱氢奎尼酸( dhq )分支点上的代谢流量，使之充分流向目的产物奎尼酸合成方向，利用基因打靶技术构建了31884宿主菌arod基因精确定位插入突变体，使dhq脱水酶( dhqase )失活，阻断了碳代谢流流向芳香氨基酸生成的方向，同时用同源重组的方法将arob基因定位整合入染色体上，解除了限速酶对碳代谢流通过共同途径到达dhq的阻遏影响，并减轻代谢负担。