大苞栝楼 meaning in English
trichosanthes bracteata voigy
trichosanthes bracteatavoigy
Examples
- The detection of agropine and trichosathin was conducted and proved available in the resulted hairy roots . r1000 remained the best alternative to r1601 though it had lower transforming capability
R1000也能比较有效的诱导大苞栝楼的发根，诱导活性却比r1601低。 - The transformed hairy roots were detected for agropine to make sure the transformation was successful . a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ) . od600 = 0 . 7 was the optimum infection concentration for the tested transforming agent , with temperature at 25 , ph at 6 . 0 , 20min of infection and 48h of co - culture and phytohormone at 1 . 0mg / l being the most favourable hairy root - inducting pattern
通过实验和对结果的数据分析，得出下面的结论：发根农杆菌r1601是较好的大苞栝楼发根的诱导菌株，它对预培养24h的大苞栝楼子叶外植体具有最好的转化效率和发根诱导率( 60 ) ，最适的菌株感染浓度od _ ( 600 )为0 . 7 ，温度27 、 ph值为6 . 0 、 20min的感染时间和48h的共培养，以及黑暗条件和1 . 0mg / l的激素最有利于发根诱导的环境条件。 - Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants , agrobacterium rhizogenes , affecting factors and their magnitude and the underlying mechanism in the transformation , therefore to determine the optimum explants , pre - culture time , tested strain concentration , infection time , co - culture time , affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test , this project was launched . with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants , agrobacterium rhizogenes r1601 and r1000 as infection agents , transformation was performed using different methods and under various circumstances
为了建立一种有效的诱导大苞栝楼发根的方法及其发根培养体系，以适合于发根生物反应器扩大培养研究和应用基础的需要；本研究对诱导发根的外植体在发根农杆菌和环境因子等影响作用下进行发根的诱发及其可能的机理进行探讨，从而找出用于发根诱导和培养的最佳外植体、预培养时间、发根农杆菌浓度、感染时间和共培养时间、诱导条件，并通过正交试验得出优化的诱导条件。