大凉疣螈 meaning in English
tylototriton taliangensis
Examples
- The homology of deduced bdnf sequences is over 90 % among the three reptiles . but the bdnf sequence of tylototriton taliangensis with those of other three reptiles showed a little lower homology . finally , the homology of bdnf gene among the reptiles and the aves is higher than that among reptiles and in other species
此外， 4个基因编码区存在着高度的同源性，除大凉疣螈同源性偏低外，另外3种bdnf基因同源性高达90 ，并且其中的爬行动物bdnf基因与鸟类的同源性高于其它物种。 - The full coding regions of bdnf genes were amplified from the genome of tylototriton taliangensis , phrynocephalus hongyuanensis , japalura splendida and cyclophiops major , respectively by pcr with the primers designed on the sequence of human bdnf gene . the pcr products were cloned into the vector pucis of esherichia coli . sequence analysis showed that the coding regions of three reptiles are the same ( 741 bp ) in length and these bdnf genes encode a peptide of 246 amino acid residues while that of tylototriton taliangensis is 744 bp in length and encodes a peptide of 247 amino acid residues
根据已有的人bdnf结构基因的全长序列设计了一对引物，利用pcr技术分别从大凉疣螈( tylototritontaliangensis ) 、红原沙蜥( phrynocephalushongyuanensis ) 、丽纹龙蜥( japalurasplendida )和翠青蛇( cyclophiopsmajor )的基因组dna中扩增到目的dna片段，并将其分别克隆到大肠杆菌载体中，然后对所获得的阳性克隆进行测序。