复制区 meaning in English
dna
duplicate field
Examples
- Bioassay showed that the toxic protein appeared high insecticidal activity against plutella xylotella larvea . lc50 was 0 . 633g / ml
序列测定结果表明该复制区为5273bps ，已在genbank注册， accessionnumber为ay278324 。 - Construction of a resolution vector with a small plasmid origional replion a small plasmid original replion about 2062bp come from b . thuringiensis subsp . kurstaki strain ybt - 1520 and the res site of tn4430 were used to construct a resolution vector
利用小质粒复制区构建解离载体利用tn4430的解离区和来源于库斯塔克亚种大小为2062bp的小质粒复制区ori2062构建解离载体pbmb1125 。 - A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。