基因灭活 meaning in English
inactivation of gene
Examples
- Thirdly , the hyl gene was cloned to puc19 and pbr322 respectively . then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro . transformed the recombinated vectors into s . equi by electro - operation , then plating on hag solid medium containing ampicillin , and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination
( 1 )直接插入外源基因灭活hyl方法：将hyl片段与载体puc19 pbr322连接后，用来自puc19的amp ~ r抗性基因片段从hyl基因的中部插入将载体上的hyl片段分为两部分； ( 2 )用hasa替换hyl部分片段的方法：将hyl片段与载体pucl9 pbr322连接后，用hasa片段替换部分hyl ，再将amp ~ r片段接入到hasa上游。