固氮酶活性 meaning in English
nitrogenase activity
Examples
- Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments . the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose , sucrose , malic acid , mannitol ) , propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum , at a ph range of 4 to 8 . 5 , at a saline concentration range of 0 . 01 % to 1 . 5 % ; low nlv " concentration had little effect on its nitrogenase activity . ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株对环境因子的适应性研究:无氮培养条件下,测定温度、碳源、酸碱度、渗透压对w12生长及固氮能力的影响,结果表明,在15 - 40下均能生长并表达固氮酶活性,其最适生长及固氮的温度为25 - 35 ;能利用葡萄糖、蔗糖、苹果酸、甘露醇等多种碳源生长并固氮,当培养基中同时存在蔗糖和苹果酸时,细菌生长和固氮活性最强;在偏酸和偏碱的条件下( ph4 . 5 - 8 . 5 )均能保持较强的生长势和较高的固氮酶活性,并能通过调节自身代谢平衡并适应环境的酸、碱性变化,使培养液趋于中性:能耐受较高的渗透压,培养液中卜、 5 naci浓度对其生长和固氮酶活性影响不大,当naci浓度升至2时,菌株的生长势及固氮酶活性才有所下降:低浓度的铰对其固氮酶活性影响不大,在0 - It has been 40 years since dobereiner and ruschel isolated the nitrogen - fixing bacteria from the rhizosphere of sugarcance plants and demonstrated the potential of diazotrophs to associate with graminaceous plants . more recent evidence of significant biological nitrogen fixation in economical important graminous species , particularly sugar cane , rice and forage grasses , has induced tremendous interest in ni fixation by non - legumes
本研究分离、筛选得到一株固氮酶活性高且稳定,生长势强的联合固氮菌株,并对其分类地位、形态及生理特征、对环境的适应性及其对植物的促生效果和作用机理作了系统研究,得结果如下。 - The nifh sequence similarity between isolates t7 and paenibacillus odorifer was 97 % . the 7 isolates with nifli gene were tested for their nitrogenase activity using acetylene reduction assay ( ara ) . isolates c4 , g2 , t7 and w5 showed nitrogenase activity , whereas nitrogenase activity was not detected in the isolates c5 , gl and t1 and suitable conditions for demonstrating nitrogenase activity for them remain to be found
采用乙炔还原方法对这7个菌株的固氮酶活性进行了测定,其中4个菌株具有固氮酶活性,菌株g2和t7的固氮酶活性相对来讲比较高,分别达到660 . 0nmol乙烯mg蛋白和292 . 5nmol乙烯mg蛋白,但用同样方法却没有测出菌株g1 、 t1和c5的固氮酶活性,它们的最佳固氮条件还有待于发现。