周质 meaning in English
periplasm
Examples
- The linear periodic time - varying vibration system can be changed to a constant coefficient linear system by l tansformation
摘要线性周期时变齐次系统的状态方程经李亚普诺夫变换后，周质系数线性时变振动系统转化为一非齐次定常线性系统。 - In the second method , the protein is secreted across the cytoplasmic membrane using the signal peptide method and then two periplasmic helper proteins facilitate the movement of the protein across the outer membrane
第二种方法是用信号肽方法分泌到细胞膜外，然后两种周质帮助蛋白促进蛋白移出外膜。 - 5 . expression and detection of soluble mg7 scfv the positive dones possessing apparent inhibitory effect were individually transfected into eco / / hb2151 ; after induction by iptg , perplasmic extracts was prepared from the induced transfectant ; the yields of soluble mg7 scfv produced by the transfected eco / / hb2151 were evaluated via immunodoting assay
7 u mg7scf64kilsrj将上述竟争eu趴中筛蹦u的mo重组咱箔列莉b体阳性克隆感染hbzb2151大肠杆菌，经iptg诱导表达可溶11mg7schv ：牛愤iptgm菌的周质提取物。 - Based on the structure and function analysis of hirudin , a potent thrombin inhibitor , and some platelet aggregation inhibitors , which contain the recognition sequence argglyasp as their functional motif , two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus . these chimera genes were constructed by pcr and inserted into the expression vector pet21a , the constructs were confirmed by restriction enzyme digestion and dna sequence analysis . these recombinant plasmids were transformed into
经限制酶消化和dna序列分析，证明两种重组质粒与设计完全一致。由于rgd -水蛭素嵌合基因上游连接了金黄色葡萄球菌蛋白a spa的信号肽序列，在iptg诱导下两种嵌合分子都获得了分泌表达，表达产物主要集中在细胞周质空间。 - The expressed fusion protein occupied more than 20 % of total bacterial protein . the fusion protein induced mainly existed in the insoluble inclusion body of the cell , only little parts of fusion protein expressed in the cytoplasm , excreted into periplasm and secreted into the cultural medium as soluble protein . through ultrasonic treatment at low temperature , soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表达产物在细胞内外的精细定位研究表明，融合蛋白cbd - lt 27在经诱导的大肠杆菌中表达量占总菌体蛋白的20以上，融合蛋白主要以不溶性包涵体的形式存在于细胞中，少量以可溶产物的形式存在于细胞质、分泌于细胞周质间腔及培养基中。