后沉淀 meaning in English
after precipitation
after-precipitation
post precipitation
postprecipitation
Examples
- Break of oil
油加热后沉淀 - After washing with reagent ( 50mmol / l ph8 . 0 tris - hcl , 100mmol / l nacl , 0 . 5mmol / l edta , 2mol / l carbamide , 0 . 2 % triton x - 100 , 0 . 2 % doc ) , ultrasound crushing ( 200 w , 150 times , 3 seconds per time , spacing 3 seconds ) and freezing - melting methods , we got hng fusion protein and m - insulin fusion protein with purity of above 80 % . 4 . radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0 . 5 unit per litre bacterial liquid
3 .表达产物初步纯化仁gly ”一flumanin融合蛋白和人胰岛素突变体融合蛋白包涵体的洗涤方案为: lj ]体沉淀溶于含zmol , / l尿索洗涤液,超声( 200w 、巧o次、 3秒/次、间隔3秒) , 4 、 i000orpm离心15min后沉淀用洗涤液充分重悬,一2 ( )冻存过夜,次日融化后4 、 10000rpm离心15min ,取上清,即得到初步纯化的融合蛋白,可去除土要的杂蛋白,融合蛋白溶解于上清液中,纯度达到80 %以_ 1 : 。