合成引物 meaning in English
synthetic primer
Examples
- The cdna of preprotein and mature protein of rabbit hypophysiscerebri was obtained . the cdna of mature growth hormone was cloned in pet - 3a , then the recombinant expression vector was transformed into
从兔脑下垂体中的mrna中,利用合成引物逆转录pcr ,获得了前体和成熟生长激素的cdna ,成熟生长激素的cdna克隆在pet - 3a表达载体并转化到大肠杆菌bl21 de3 plys中,获得了高效表达,表达量达到大肠杆菌总蛋白的40 % 。 - To explore the feasibility of ib edible vaccine , we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product . the findings are as follows : 1 . a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121
为了探索ibv可食性疫苗的可行性,我们进行了转基因马铃薯表达ibv免疫原基因及其表达产物免疫原性的研究,取得以下结果: 1根据周继勇等报道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表达载体的多克隆位点,设计并合成引物,以含s1pbs质粒为模板扩增s1基因,将扩增片段定向克隆到pbi121质粒的35s启动子下游。