变性聚丙烯酰胺凝胶 meaning in English
denaturing polyacrylamide gel
Examples
- The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels . numerous and distinct bands could be detected by silver staining
Pcr扩增产物经6变性聚丙烯酰胺凝胶垂直电泳分离后，银染能检测到多而清晰的条带。 - The recombinant vectors were transformed into e . coli m15 respectively and the expression was induced based on the optimal values of the iptg concentration incubation temperature and induction time determined in the previous section
根据优化确定的iptg诱导浓度、诱导温度和时间进行诱导表达。 5的积层胶， 15的分离胶，变性聚丙烯酰胺凝胶电泳( sds - page )检查表达情况。 - Method dna of 58 unrelated individuals was supplied by no . l hospital of shanxi medical university and extracted by phenol - chloroform method . after pcr , the sequencing fragments were generated and analysed using drhodamine terminator cycle sequencing ready reaction kit and abi 377 respectively
将上述58名无关个体的mtdna扩增后， 10非变性聚丙烯酰胺凝胶(含10甘油)电泳，银染，进行sscp分析。 - Method to collect respectively 180 unrelated males " venous blood 500ul , who lived in shanxi province , 120 unrelated mongolians " venous blood 500ul , who lived in the inner mongolia autonomous region , and the blood is anticoagulant with edta , then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法采集180例山西汉族和120例内蒙古蒙古族男性无关个体静脉血各500ul ， edta抗凝，用tkml液反复洗涤至无色，加入2蛋白酶k缓冲液180ul ，蛋白酶k ( 20mg ml ) 20ul ，在56消化至液体清亮为止，用酚-氯仿法抽提dna ， pcr扩增dys413位点， 6非变性聚丙烯酰胺凝胶电泳， 1硝酸银染色分型。 - . moreover , some samples appeared several active bands in the gel , which indicated the existence of different types of sod or multi - subunits of sod in these samples . the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella , which observed after the gram ' s staining and flagellum staining
同时，利用非变性聚丙烯酰胺凝胶( page )电泳后的凝胶显色反应，发现一些样品出现了多条活性带，这可能是因为在这些细菌提取物样品中含有不同类型的sod分子，或是同一类型的sod含有多个亚基组成。