反转录pcr meaning in English
reverse transcriptional pcr rt-pcr
rt-pcr
Examples
- G gene of rabies virus m , the of two main regions ( about 1000nt ) , ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m , isolated from mouse in he nan , china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m . these regions were sequenced by the produce of pcr directly . comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2 . 5demo software
本研究对我国河南某地野鼠体内分离的狂犬病野毒株mrv基因的3161位? 4162位( 1001个碱基)和4012位? 4863位( 851个碱基)片段进行了反转录pcr扩增和序列测定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析软件，与已发表的代表性毒株g基因全序列进行核苷酸和氨基酸序列的比较分析，结果表明在同一基因型中， mrv和国际标准攻毒株cvs的同源性最高( 96 . 5 ) ，和中国减毒株ctn的同源性最低( 79 . 8 ) 。