分子筛层析 meaning in English
sieve chromatography
Examples
- Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction , fraction with ( nhi ) 2864 , hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr . cml agglutinated type a human red blood cells specially . the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis
野花生豆( crotalariamucronata )经磨粉、浸取、硫酸铵分级沉淀、猪胃粘蛋白- sepharose4b亲和层析、 sephacryls - 200hr分子筛层析可得到一表观分子量为103kd且对a型血红细胞专一凝集的野花生豆凝集素( crotalariamucronatalectin ， cml ) 。 - A man / glc - specific dolichos puepureus lectin ( dpl ) was purified from seeds of dolichos puepureus l . var . by a procedure including precipitated by ammonium sulphate , deae - 52 iron - exchange and sephacryl s - 300 hr gel filtration . the purified dpl showed a single band on page and ief gel , two bands on sds - page under reducing and non - reducing congditions
黑皮扁豆( dolichospurpureusl . var . )经粉碎、浸取、硫酸铵分段盐析、 deae - 52离子交换和sephacryls - 300hr分子筛层析可得到一表观分子量为155kd的甘露糖葡萄糖专一的黑皮扁豆凝集素( dolichospurpureuslectin ， dpl ) ，无血型专一性。 - After the acet is vaporized , the active substance in water is gotten . and which is vaporized at low temperature . then the crude active substance is purified by column chromatography on sephadex g - 75 . after a series of purifications again , we could get some white powder at last . though the active substance is diluted to50 g / ml , the activity is still checkeded - up through phyto phtnora casicileon . the purified active substance is insensitive to heat , resistant to chloroform 、 ethanol and the orhers . in addition , the active substance is sensitive to high ph ( 10 ~ 14 ) , but it is not sensitive to low ph ( 1 ~ 5 ) . furthermre , when the ph is made to low again , the activity of it ' s comes back
用蒸馏水对菌体稀释；加入适量吸附树脂在150rpm 、 28下振荡吸附4h ， 80 %的丙酮解吸，过滤解吸液得到活性物质的澄清溶液，旋转蒸发仪旋转蒸发去处丙酮，经sephadexg - 75分子筛层析得单一活性峰，收集峰值部分样品液经冷冻干燥得到淡褐色粉末，该活性物质用丙酮充分洗涤、甲醇-乙醚重结晶获得略带微黄的白色粉末，该活性物质50 g / ml仍可对苏云金芽孢杆菌hd - 1产生明显的抑制作用。 - Then enzyme was purified with a deae - cellulose ( 5 . 5x50cm ) column , a toyopearl hw - 65 ( 5 . 5 x 50cm ) column and a sephadex g - 200 ( 5 . 5 x 80cm ) column . finally , the enzyme was purified for 10 folds with the recovery of 17 . 4 % . page showed a single band for the purified creatinase
3 、肌酸水解酶的提纯酶在硫酸铵饱和度为40 80之间完全沉淀，先后经过deae - cellulose离子层析柱、 toyopearlhw - 65疏水层析柱、 sephadexg - 200分子筛层析柱层析，最终使酶提纯10倍，最终得率为17 . 4 。 - We have sifted 103 medicinal plants , roughly identified 17 plants might contain antifungal proteins . antifungal protein was purified from cassia sophera linn , by extraction , fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ) , cation - exchange chromatography of cm - sepharose ff xk 26 , the first cation - exchange chromatography of mono s and the second one , followed by gel filtration of superose 12hr
对茳芒决明进行了抗菌蛋白的分离纯化：经粉碎、磷酸缓冲液浸提、硫酸铵沉淀、 cm - sepharoseffxk26阳离子交换层析、两次monos阳离子交换层析、 superose12hr分子筛层析可得到具抗真菌活性的蛋白。