凝胶过滤层析 meaning in English
gel filtration chromatography
Examples
- At last , we got the pure protein with the determination of electrophoresis . the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis . the isoelectric point is 8 . 6 ? . 3
经sds凝胶电泳和凝胶过滤层析两种方法测定该酶的分子量分别为33113dalton和28840dalton ；其等电点pi = 8 . 6 0 . 3 。 - [ methods ] consideration of free heparin , unlike polypeptides , adsorb poorly onto plastic surfaces . in order to study the interaction of heparin with ifn - y using elisa assay , heparin - bovine complex ( hbc ) was synthesized by conjugating heparin to bsa in the presence of sodium cyanoborohydride
结果凝胶过滤层析获得了高纯度的hbc ，通过hbc将肝素包被在酶标板上， ellsa实验表明， ifn一y主要与hbc中的肝素结合( p ( o ， 01 ) 。 - The separation of the formica rufa linnaeus samples by pel filtration chromatography . in this part , the author has primarily separated the formica rufa linnaeus samples by glucosan gel g - 50 filtration chromatography . the principle is the difference of the molecular weight of different components
红褐林蚁样品的凝胶过滤层析分离本部分旨在通过葡聚糖凝胶g - 50过滤层析初步分离红褐林蚁样品，根据分子量大小不同而达到分离目的。 - The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp . the fermentation liquor also showed good dehairing activity . the alkaline protease ( named dhap , dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography , ion exchange and gel filtration
通过cm - sepharosefastflow离子交换层析， deae - sepharosefastflow离子交换层析， sephacryls - 100 ， sephacryls - 200凝胶过滤层析，疏水层析等纯化步骤对短小芽孢杆菌发酵液中的碱性蛋白酶进行了纯化。 - Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae , extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates . the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents . a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用线虫诱导下owvt - 1菌株液体发酵，通过粗分级分离、离子交换层析和凝胶过滤层析分离提纯了一个分子量为31kda的丝氨酸蛋白酶，生物学测定表明其对大豆胞囊线虫二龄幼虫具有致死作用，同时测定了该酶理化特性，酶活力在75附近酶活力最高，随着ph的增加酶的稳定性升高，与胆碱酯酶具有相似的ph曲线，对特异性底物aape ( suc - ala - ala - pro - glu - pna )具有作用， ssi和ci - 2抑制该酶的活性。