共转染 meaning in English
cotransfection
Examples
- The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
另外，通过共转染和筛选纯化获得了携带appa基因的重组家蚕杆状病毒。 - To check if human rap 1 gap can form dimer in mammalian cells , pcdna - myc - raplgap ( human ) and pmt2 - ha - raplgap ( human ) were constructed and co - transfected into a14 cells
为解决这个问题，我们构建tpcdna3llmyc rp1gap和pmtz ha raplgap重组质粒，将其共转染入ai4细胞。 - We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker
在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。 - In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 - To investigate the consequence of this interaction , aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector . confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus
在构建了红色荧光蛋白aes表达载体后，将其与tle绿色荧尤蛋白载体共转染细胞，共聚焦显微镜观察发现这两种分子在胞浆中有共存现象，而且aes的表达可抑制tlei向胞核内的聚积。