共转化 meaning in English
cotransformation
Examples
- In y2h , rapgap from c . elegans was used as bait to screen c . elegans cdna library . after - lth screen , 63 colonies were checked
在酵母双杂交系统中，将ppc97 rpg ”与ppc86七dna文库共转化入酵母菌y190细胞，经过一lth筛选后，有63个菌落拟为阳性菌落。 - Multi - genes co - transformation is very important for studying on metabolic pathway which were controlling by multi - genes and generation maker - free transgenic plants
摘要实现多基因在同一受体植株中的共转化在研究植物多基因控制的代谢途径和获得去选择标记转基因植株中具有巨大的应用前景。 - This article summarized the advance of the co - transformation research in recent years , especially expatiated the new types , mechanism , effect factors and application foreground of agrobacterium mediated co - transformation systems
笔者综述了目前国内外在共转化上的研究进展，尤其对农杆菌介导的共转化系统的新类型、作用机理、影响因素及其应用前景进行了阐述。 - When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a , 109 candidates interacting with nifa had been selected by testing for the expression of the his3 , ade2 and lacz reporter genes
诱饵质粒和文库质粒共转化酿酒酵母( saccharomycescerevisiae ) pj69 - 4a ，通过检测报告基因his3 、 ade2及lacz的表达进行筛选，初筛得到109个阳性酵母菌落。 - 11 interacting candidates proved to be the positives after isolating the mixed plasmids from yeast and recotransforming each purified ad / library plasmid and pgbd - nifa into s . cerevisiae pj69 - 4a respectively . the genes of the 11 positives had been sequenced and 4 different gene fragments were obtained
分别对其中的ad / library质粒进行分离纯化，将每个纯化质粒再次与诱饵质粒共转化酵母pj69 - 4a进行验证，得到11个阳性克隆，测序结果显示其中有4个不同的基因片段，分别命名为s4 、 s35 、 s64和s65 。