共转 meaning in English
corotation
Examples
- The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
另外，通过共转染和筛选纯化获得了携带appa基因的重组家蚕杆状病毒。 - ( 3 ) in co - revolution disc cavity , heat transfer coefficient which is high on most region of disc surface show that the co - revolution avails the heat transfer on disc surface
( 3 )在共转盘腔结构中，大部分区域盘面的换热系数都较高，表明共转状态对盘面的换热有利。 - To check if human rap 1 gap can form dimer in mammalian cells , pcdna - myc - raplgap ( human ) and pmt2 - ha - raplgap ( human ) were constructed and co - transfected into a14 cells
为解决这个问题，我们构建tpcdna3llmyc rp1gap和pmtz ha raplgap重组质粒，将其共转染入ai4细胞。 - We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker
在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。 - In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。