体外诱变 meaning in English

invitro mutagenesis

The routine method for inducing protein function evolution is imitating and speeding protein gene mutation . in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ) . the higher the concentration is , the stronger its inducing ability is in a certain range
本研究采用5 -溴脱氧尿苷三磷酸( 5 - bdu )部分取代脱氧胸苷三磷酸( dttp ) ，在pcr的过程中对克隆的野油菜黄单胞菌的-淀粉酶基因进行了体外诱变，诱变结果表明： 5 - bdu浓度越高，诱变越强；浓度越低，诱变越弱。
The new methods to identify _ amylase activity and its producing bacteria , and the preparation of high efficiency competence cell were reported . three _ amylase genes were cloned from bacillus subtilis , xanthomonas campestrisand aspergillus oryzae . two mutant genes with high activity was reported by using the methods of mutagenesis in vitro
主要研究内容包括-淀粉酶及其产生菌的快速鉴定新方法、高效感受态细胞的制备、 -淀粉酶基因的克隆和-淀粉酶基因的体外诱变等四个主要方面。