亲本株 meaning in English
parent plant
Examples
- In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株,在通用prv转移载体pbdtk - uni的基础上,在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒,同时将下游同源臂增加了一个1 . 7kb的kpni片段,使上下游同源臂的长度都超过了1kb ,构建了一个新的转移载体puni - lacz 。