亲和吸附 meaning in English

affinity adsorption

The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。