交错延伸 meaning in English
stagger extension process
Examples
- It clearly allows us to engineer enzymes with novel functions and features by strategies , such as error - prone pcr , dna shuffling and stagger extension process ( step ) , when very little is known about spatial configuration of proteins . therefore millions of years in nature can in principle be accomplished in the test within several years . according to the basic idea of directed evolution , we modified the a - aldc gene by error - prone pcr
它不需要事先了解酶的空间结构和催化机制，而通过在体外模拟自然进化机制(随机突变，重组和自然选择) ，利用易错pcr ， dna改组，交错延伸等方法，改造酶基因，定向选择(或筛选)有价值的非天然酶，短期内在试管中完成在自然界需要几百万年的进化过程。