亚基因组 meaning in English
subgenome
Examples
- After transcription , we gained the subgenomic rna that may play the role of the template rna recognized by den rdrp
经转录反应获得den亚基因组rna ，即为denrdrp可特异识别的模板rna 。 - ( 2 ) assay of rdrp activity and immunoreactivity of the recombinant ns5 first we respectively cloned the 5 " and 3 " terminal region ( tr ) sequence of the viral genome by rt - pcr and jointed the two segment by ligation
( 2 )重组nss蛋白rdrp活性和抗原性的分析:通过rt一pcr分别获得den基因组5 ’末端区( tr )和3 ‘一r的cdna片段，将两片段连接，形成两端与原病毒基因组trs序列相同的亚基因组dna 。 - Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china . cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively . total rna extracted from field samples were used for rna dot - hybridization
用侵染马蹄莲的dsmv3末端序列和黄瓜花叶病毒( cmv )的亚基因组启动子区互补dna序列为标记探针，对自然感病的天南星科植物进行rna斑点杂交，并结合双链rna分析、病毒提纯和形态学观察，对杭州等地16属天南星科植物的81个样品进行了病毒鉴定。 - Secondly , we established a rdrp reaction system . then the recombinant ns5 protein was added to the system and its rdrp activity was confirmed after the newly synthesized rna showed resistant to rnase a . furthermore , we found that the recombinant ns5 could specifically bind antibodies in anti - den serum indicating the immunoreactivity of the recombinant ns5 and the irnmunogenicity of den ns5
重组nss蛋白的rd即反应和反应产物的rnasea敏感性实验表明，所得重组nss蛋白具有良好的rdrp活性，能以亚基因组rna为模板合成与之互补的rna ，新生链是与模板链相结合的双链形式存在于rd即反应体系中。